ABSTRACT
Objective: To investigate the effects of silencing nuclear division cycle 80 (NDC 80) gene expression on the proliferation, migration and invasion abilities of human gallbladder carcinoma GBC-SD and NOZ cells. Methods: Real-time fluorescent quantitative PCR and Western blotting were used to detect the expressions of NDC80 mRNA and protein in gallbladder carcinoma GBC-SD and NOZ cells after the transfection of NDC80 siRNA, respectively. After NDC80 gene silencing, the proliferation of GBC-SD and NOZ cells were detected by CCK-8 test and clone formation test, respectively. Transwell chamber assay was used to detect the migration and invasion abilities of gallbladder carcinoma cells after NDC80 gene silencing. Results: The NDC80 siRNA (si-NDC80 group) and the negative control (si-NC group) were successfully transfected into gallbladder carcinoma GBC-SD and NOZ cells, respectively. The expression levels of NDC80 mRNA and proteins in GBC-SD and NOZ cells transfected with si-NDC80 were significantly lower than those in si-NC group (all P < 0.001). Compared with the si-NC group, si-NDC80 transfection reduced the proliferation and clone formation abilities of GBC-SD and NOZ cells (all P < 0.001), and the migration and invasion abilities of GBC-SD and NOZ cells transfected with si-NDC80 were significantly decreased (all P < 0.001). Conclusion: Silencing the expression of NDC80 gene can inhibit the proliferation, migration and invasion of human gallbladder carcinoma cells, suggesting that NDC80 plays an important role in the occurrence and development of gallbladder carcinoma.
ABSTRACT
Cholecystectomy is currently the most important surgical treatment for gallbladder disease, however, the complications of cholecystectomy, especially the occurrence of intestinal tumors has gradually aroused attention of scholars. In recent years, scholars have found that bile acid metabolism changes significantly after cholecystectomy, which may be the main cause of colorectal cancer induction, but the specific mechanism of colorectal cancer induced by cholecystectomy has not been clarified. In this article, the changes of bile acid metabolism after cholecystectomy (the size and composition of bile acid pool) and the mechanism of colorectal cancer induction after cholecystectomy were summarized and discussed based on the research progress at home and abroad, aiming at providing theoretical basis for the exploration of the prevention and treatment of colorectal cancer.